Nonparametric analysis of replicated microarray experiments

Nonparametric analysis of replicated microarray experiments

Nonparametric Approaches to Detecting Differentially Expressed Genes in Replicated Microarray Experiments

MOTIVATION An important goal in analyzing microarray data is to determine which genes are differentially expressed across two kinds of tissue samples or samples obtained under two experimental conditions. Various parametric tests, such as the two-sample t-test, have been used, but their possibly too strong parametric assumptions or large sample justifications may not hold in practice. As altern...

MArray: analysing single, replicated or reversed microarray experiments

UNLABELLED MArray is a Matlab toolbox with a graphical user interface that allows the user to analyse single or paired microarray datasets by direct input of the raw data output file from image analysis packages, such as QuantArray or GenePiX. The application provides simple procedures to manually evaluate the quality of each measurement, multiple approaches to both ratio normalization (simple ...

Replicated Microarray Data

Replicated Microarray Data

cDNAmicroarrays permit us to study the expression of thousands of genes simultaneously. They are now used in many different contexts to compare mRNA levels between two or more samples of cells. Microarray experiments typically give us expression measurements on a large number of genes, say 10,000-20,000, but with few, if any, replicates for each gene. Traditional methods using means and standar...